Title: Study on inhibitory and mechanism of aspirin on proliferation of Molt-4 cells in vitro
Abstract: Objective:To explore the effects of aspirin on proliferation of T-acute lymphoblastic leukemia cells lines Molt-4 in vitro.Method:Molt-4 cells were treated with aspirin at different concentrations in culture.Growth inhibition was detected by MTT assay,the changed cell cycles distribution of Molt-4 cells induced by aspirin was analysed by flow cytometry.The expression of β-catenin and cyclinD1 were detected by SYBR green i-based real-time quantitative RT-PCR.Result:Aspirin treatment caused a dose-dependent inhibition of Molt-4 cell proliferation,with a 72h IC50 of 1.58mmol/L.Aspirin could commol/Lit Molt-4 cells to G0/G1 phase arrest.At the same time,aspirin treatment induced a decrease in the expression level of CyclinD1 mRNA and expression level of cyclinD1 protein.Conclusion:Non-steroidal anti-inflammol/latory drugs aspirin treatment could inhibit proliferation of Molt-4 cells by decreasing abnormal expression of cyclinD1.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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