Title: Determination of chlorogenic acid,baicalin,luteolin-7-O-glucoside in Yinhuang Oral Liquid by HPLC
Abstract: Objective To develop an HPLC method for determining chlorogenic acid,baicalin and luteolin-7-O-glucoside in Yinhuang Oral Liquid(Consisting of Flos lonicerae,Radix scutellariae).Methods Three active components were separated on an Agilent Eclipse XDB-C18 column(250 mm×4.6 mm,5 μm) with a gradient elution mode.The analytes were quantified according to their peak area detected at 350 nm.Results The calibration ranges of chlorogenic acid,baicalin and luteolin-7-O-glucoside were 1.6-80.0,4.2-208.0 and 0.08-44.0 μg·mL-1,respectively.And their contents in samples were 1.25,9.25 and 0.2 mg·mL-1,respectively.Conclusion The proposed method is simple,rapid and accurate.It might be useful in the quality control of the preparation.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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