Title: Preliminary Study on Cyclophosphamide-Mutagenesis Related-Gene with mRNA Differential Display
Abstract: Abstrcat To create a method of mRNA differential display with silver staining in studying mutagenesis of drugs, total RNA samples were extracted from Balb/c mouse liver treated/untreated with cyclophosphamide, and were used as templates in differential display PCR.To use dT_(12)CG,dT_(12)AG and dT_(12)GG as the anchored primers, reverse transcription and PCR were proceeded differently.After running on 6% denaturing PAGE gel, differentially expressed bands were excised and recovered, and finally reamplified. These reamplified bands were cloned, sequenced and searched by Blast.Two new gene fragements were discovered. These new gene fragements lay a foundation to study mutagenesis.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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