Title: Enhanced sensitivity to cisplatin in human gastric carcinoma cell line SGC7901 by the downregulation of the survivin gene with siRNA technology
Abstract: Objective To detect the variations in cisplatin sensitivity of human gastric carcinoma cell line SGC7901 before and after transfection to specifically downregulate the expression of surviving gene with small interfering RNA(siRNA) technology.Methods This experiment is divided into the control group,cisplatin group(CDDP group),lipsome group(lip group),siRNA group,transfection group and siRNA combined CDDP group(combined group).Survivin siRNA was artificially synthesized and then transfected into gastric cancer cells with liposome.The transfection effects were determined by fluorescence microscopy.The inhibitory rate of cell proliferation was assayed by MTT,and the expression of survivin protein was determined by Western blot and immunocytochemical staining.The morphological changes of the apoptotic cells were observed by Hoechst staining.Results As observed under the fluorescence microscope after Hoechst staining,the nuclei presented as normal blue in the control group,lip group and siRNA group,while CDDP group,siRNA transfection group and siRNA combined cisplatin group had such changes as reinforced nuclear staining,karyopyknosis and karyorrhexis with apoptotic changes.The inhibition rate of cell proliferation in siRNA combined cisplatin group(63.93±4.22)% was markedly higher than the other groups(P0.05).Compared with other groups,the expression of survivin protein in the transfection group and siRNA combined cisplatin group was significantly reduced,and the differences had statistical significance(P0.05).Conclusion The application of siRNA technology to downregulate the survivin gene expression of human gastric carcinoma cell line SGC7901 is able to enhance its drug sensitivity to cisplatin.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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