Title: Effect of Niaodu Qing Capsule and Its Separated Prescriptions on Renal TGFβ_1 mRNA Expression in Rats After 5/6 Nephrectomy
Abstract: Objective To observe the effect of Niaodu Qing Capsule(NQC) and its separated prescriptions on renal transforming growth factor β1(TGF-β1)mRNA expression in rats with chronic renal failure(CRF).Methods SD rat models of CRF were induced by 5/6 nephrectomy,and then the CRF rats were randomized into model group,Huangqi(Radix Astragali,1.5 g·kg-1·d-1) group,Danshen(Radix Salviae Miltiorrhizae,1 g·kg-1·d-1) group,Dahuang(Radix et Rhizoma Rhei,1 g·kg-1·d-1) group,Huangqi Danshen Dahuang(1 g·kg-1·d-1) group,NQC group(1 g·kg-1·d-1).Except the model group,the rats in other groups received gastric gavage of corresponding medicine according to the experimental design.After treatment for six weeks,the levels of blood urea nitrogen(BUN) and serum creatinine(SCr) were detected,and the expression level of renal TGF-β1 mRNA was examined by reverse transcription polymerase chain reaction(RT-PCR).Results The levels of BUN and SCr were decreased in the medicated groups(P0.05 or P0.01),and the effect on decreasing SCr level in NPC group was superior to that in Dahuang group(P0.05).The expression of TGF-β1 mRNA was shown in all of the rats,and the expression was obvious in the model group,and mild in NQC group and Huangqi Danshen Dahuang group.Conclusion The therapeutic mechanism of NQC for CRF is related with the down-regulation of renal TGF-β1 mRNA,and the effect of the compound prescription of NQC for CRF is superior to that of the single herbs of NQC.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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