Title: Curcumin inhibits the proliferation of rat airway smooth muscle cells
Abstract: Objective To investigate the effect of curcumin on the proliferation of airway smooth muscle cells(ASMCs).Methods Primary ASMCs of rats were cultured.The absorbance(A450) value of ASMCs with treatment with platelet-derived growth factor(PDGF) in the presence or absence curcumin were detected by CCK-8 kits to abserve the proliferation induced by PDGF and the antiproliferation induced by curcumin.The levels of ERK1/2 protein were determined by western blotting.ResultsCompared with control group(1.04±0.12), the absorbance(A450) value of 10 ng/ mL PDGF group(1.16±0.14) and 50 ng/ mL PDGF group(1.30±0.15) both were increased significantly after treatment with PDGF at 2 days(P0.01).After incubation with curcumin at 12, 24 and 36 hours, compared with control group, the average cell inhibition rates of 11.25 μmol/L group(21.57%, 43.55% and 65.99%), 16.88 μmol/L group(39.64%, 57.44% and 77.80%) and 25.31 μmol/L group(44.50%, 53.68% and 92.68%) were increased significantly(P0.05).The levels of ERK1/2 protein were significantly decreased at 20, 40 and 60 minutes after treatment curcumin(25 μmol/L) with PDGF(25 ng/mL).ConclusionsThe proliferation of ASMCs was inhibited by curcumin, and this may be ralated to inhibition of activation of ERK signaling pathway.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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