Title: Effect of water extract from mycelium of Cordyceps sinensis on apoptosis of SP2/0 cells
Abstract: MTT assay,agarose gel electrophoresis,flow cytometric analysis and Hoechst33342 staining were performed to study apoptosis and its mechanism.After SP2/0 cells were treated with water extract of mycelium,growth of SP2/0 cells was inhibited and SP2/0 cells appeared lighter green in fluorescence microscope.A typical DNA ladder was detected by agarose gel electrophoresis of DNA extracted from SP2/0 cells.Flow cytometric analysis of SP2/0 cells by Annexin-V-Fluos and propidium iodide double staining demonstrated that quantity of apoptosis cell increased with water extract induction.The water extract of mycelium induced apoptosis in SP2/0 cells,indicating that growth of SP2/0 cells was inhibited.The mechanism of anti-tumor activity of water extract of mycelium may be related to induction of apoptosis in tumor cells.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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