Title: Effect of Humic Acid on Tubers Secondary Metabolism and Growth Physiological Property of Pinellia pedatisecta under High Temperature Stress
Abstract: Humic acid is a kind of structure,material of a similar nature of complex compound.At the same time,it is a kind of hydrophilic colloid,and with a variety of groups.We conducted an experiment to investigate the effect of HA on physiological indexs and secondary metabolism.We set up five different leaf spray application concentration gradient(0,20,40,60,80,100 mg/L)of HA.Using potted trial,we analyzed the influence of the physiological indexes by HA on Pinellia pedatisecta seedlings under high temperature stress,as well as the regulation effect of secondary metabolism.The results showed that:(1)Each HA concentration treatment can promoted the growth of P.pedatisectain different degrees under high temperature stress.With the treated of 80 mg/L,the fresh weight of tubers,petiole and the total chlorophyll content is the highest,compared with the control has significantly increased 21.25%,118.5% and 37.19%,respectively.(2)Compared with the control,under the concentration of 60 mg/L,the content of soluble sugar,soluble protein in leaves were highest.Both respectively increased by 66.67% and 40.91%,respectively.The activities of antioxidant enzymes(SOD,POD)were the highest,with significantly increased by 818.98% and 48.2%,respectively.The content of MDA decreased significantly by 62.08%,and the content of guanosine in tuber was significantly increased by 52.94%.(3)Under 80 mg/L,the content of free proline in leaves and the content of total alkaloid,total organic acid and adenosine of tubers are the highest.And compared with the control they are significantly improved by 169.63%,27.19%,42.32% and 96.23%,respectively.Researches showed that fitting consistency of HA treatment could significantly increase the activities of antioxidant enzymes(SOD,POD)and the content of osmoregulation substance(soluble sugar,soluble protein and free proline).At the same time improved the metabolism of active ingredients contents accumulation(total alkaloid,total organic acid,adenosine and guanosine)in the tuber of P.pedatisecta seedling leaves under high temperature stress.Effectively relieve summer heat of P.pedatisecta seedling leaves damage,delay aging,improve the seedling resistance to heat,promote growth,extend the growing season.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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