Title: Role of transfected angiotensin II receptor anti-sense nucleotide in the growth of cardiomyocytes
Abstract: Objective To evaluate the role of transfected angiotensinⅡ(Ang Ⅱ) receptor AT 1 anti-sense nucleotide (AT 1A) in the expression of subtypes of AngⅡ receptor mRNA, synthesis of protein and nucleic acid in cardiomyocytes. Methods AT 1 cDNA sequence (476 bp) was cloned with RT-PCR and reversely inserted into PcDNA3.1 (5.4 kb) to construct an intact plasmid containing AT 1A (PAT 1A). The plasmid was then transfected into the cultured cardiomyocytes and identified with RT-PCR and Western blot. The synthesis of protein and nucleic acid identified by 3H-Leu and 3H-TdR incorporation, and expressions of AT 1 and AT 2 mRNA by RT-PCR, were compared between transfected and nontransfected cardiomyocytes after being stimulated with 10 -7 mol/L AngⅡ for 24 h. Results The plasmid PAT 1A were successfully constructed. The AT 1 mRNA and its protein were expressed significantly less in the transfected cardiomyocytes than in the control (P0.01). In the transfected cardiomyocytes, AT 1 mRNA expression was markedly decreased, but that of AT 2 mRNA obviously increased (P0.01) when compared with the nontransfected cardiomyocytes after stimulation for 24 h with AngⅡ 10 -7 mol/L; no significant difference was found in 3H-Leu and 3H-TdR incorporation between them. Conclusion After the cardiomyocytes was tranfected with AT 1A, the expression of AT 1 mRNA was markedly suppressed,while AT 2 mRNA up-regulated at the same time. Our results indicate that AT 1A blocking can not effectively interrupt the Ang Ⅱ-induced synthesis of the protein and nucleic acid in cardiomyocytes.
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot