Title: Anti-tumor immunity of child HepG2/hB7-1 vaccine
Abstract: Objective To assess the potential of immune-costimulating signal B7-1 vaccine in inducing anti-tumor immunity effect.Methods After HepG2 were transfected with B7-1 gene,the expression of B7-1 in cells and HepG2 cells growth were detected by flow cytometric analysis and MTT assay.The T cell function was detected by the method of ELISA.Results Flow cytometry revealed that HepG2 cells had a very low expression of B7-1 on the cell surface(positive rate:1.1%).Conversely,the HepG2/B7-1 clone strongly expressed B7-1(positive rate:18.5%,increasing 16.8 times compared to the former)on the cell surface.After transfection with B7-1 gene,the HepG2 cell proliferation slowed down,and the IFN-γ production by HepG2/B7-1 vaccine was higher than wild HepG2.Conclusion The transduction of B7-1 gene can enhance B7-1 expression in HepG2 cell,inhibit the proliferation of HepG2 cell,and activate T cell to produce IFN-γ in vitro,hence enhancing the anti-tumor immunity.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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