Title: Study of Huangqi Weimao Mixture Regulating Unbalance Cytokine in Renal Tissue of Diabetic Nephropathy Rat
Abstract:Objective: To study the effect of Huangqi Weimao mixture on glomerular VEGF,TSP-1,NO and ET-1 of diabetic nephropathy rat. To explore the treatment mechanism of Huangqi Weimao mixture on diabetic neph...Objective: To study the effect of Huangqi Weimao mixture on glomerular VEGF,TSP-1,NO and ET-1 of diabetic nephropathy rat. To explore the treatment mechanism of Huangqi Weimao mixture on diabetic nephropathy. Methods: The diabetic nephropathy model in rats was established with unilateral kidney and streptozotocin intravenously. After the success of modeling,the rats were randomly divided into sham operation group,diabetic nephropathy group,diabetic + Huangqi Weimao mixture group and diabetic nephropathy + losartan potassium group. The experiment continued for 12 weeks. We observed glomerular vascular endothelial cell growth factor and thrombospondin-1 expression by immunohistochemical staining. We also observed NO and ET-1 contents in renal tissue. Results: The VEGF had strong expression in glomerular of sham operation rats while TSP-1 was occasionally expressed. VEGF expression was decreased obviously in model rats,and the expression of TSP-1 increased significantly in model rats( P 0. 01,compared with sham operation group). VEGF expression was increased and TSP-1 expression was significantly reduced in two treatment groups and there was significant difference compared with the model group( P 0. 05,P 0. 01). There was no significant difference between the two treatment groups( P 0. 05). NO level was significantly decreased and ET-1 level was increased in model rats( P 0. 01 or P 0. 05). Huangqi Weimao mixture treatment can significantly increase NO level and reduce ET-1 level( P 0. 05). There was no significant difference compared with model group and losartan potassium group( P 0. 05). Conclusion: Huangqi Weimao mixture can increase VEGF expression and reduce TSP-1 expression in renal glomerulus and can increase the content of NO and reduce ET-1 level,regulate VEGF,TSP-1 and NO,ET-1 balance. Therefore it can protect glomerular endothelial cells,promote blood capillary and small vessel regeneration and reconstruction. At the same time it is proved to be scientific and effective of Chinese medicine. And it is also the proof of the relationship theory of Qi and blood.Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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