Title: Sensitivity comparison of SYBR Green I with EB in Q-PCR
Abstract: Aim: To compare the sensitivity of SYBR Green I stain with ethidium bromide(EB)stain in quantitative PCR(Q-PCR).Methods: The DNA samples were isolated from 26 cases of Down's syndrome and 20 normal subjects. The DNA amplification products were subjected to agarose gels with SYBR Green I stain and EB stain, respectively. The optical densitometry of the PCR products was measured.Results: DNA amplification products in 24 cycles stained with SYBR Green I were clearly visualized and quantitatively detected,but DNA amplification products stained with EB could not be quantitatively analyzed until 28 cycles.Conclusion: SYBR Green I is more sensitive and rapid for detecting DNA in Q-PCR assay compared with EB.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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