Title: Expression of H2AX in new born rats with hyperoxia induced lung injury
Abstract:Objective To explore the expression of H2AX in new born rats lung with hyperoxia-induced lung injury. Methods Eighty new born rats were randomly exposed to hyperoxia (model group ) and to room air(con...Objective To explore the expression of H2AX in new born rats lung with hyperoxia-induced lung injury. Methods Eighty new born rats were randomly exposed to hyperoxia (model group ) and to room air(control group,n = 40 each ). Lung injury was induced by hyperoxia exposure, the expression of H2AX was detected by immunofluorescence technique and Western-blot at 1, 2, 3 , 5 and 7 days after exposure. Results Average intensity of H2AX protein in new born rats of model group was increased significantly compared with the control group at the days 1,2 ,3( P0.01)and 5( P 0.05)after hyperoxia exposure, but not for the 7th day ( P0.05), H2AX average intensity decreased with the time of hyperoxia exposure going on (F=45.02, P0.01) in the model group. H2AX relative expression was consistant with the result of average intensity, increased quickly in model group and decreased graduly with the length of time exposure to hyperoia compared with the control group. Conclusions H2AX is closely related to the development of hyperoxia induced lung injury probably.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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