Title: Genetic diversity of cultivated Luohanguo ( Siraitia grosvenorii )based on ISSR marker
Abstract: Luohanguo( Siraitia grosvenorii ),a liana species of Cucurbitaceae,is locally economic important cultivated plant,endemically distributing in Southern,China. The genetic diversity was analyzed using inter simple sequence repeat(ISSR)technique,that a total of 62 female from eight cultivars and 13 male individuals were collected. Employed with 13 ISSR primers,88 scorable amplified bands were detected,of which 64 were polymorphic. Principal components analysis(PCA)was conducted for females and males respectively,and dendrogram was constructed using an unweighted pair group method with arithmetical averages(UPGMA)method and Nei Li similarity coefficient. The results demonstrated that a high degree of similarity among the major cultivars Qingpiguo,Hongmaoguo and Baopengzi,with 0.958,0.952 and 0.988 of mean similarity coefficient of intra cultivars respectively,which indicated that they were low genetic diversity. However,the cultivars Chashanguo and Dongguahan showed a relatively high genetic diversity,with mean similarity coefficient of 0.879 and 0.829. The male individuals also had a high level of genetic diversity.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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Cited By Count: 2
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