Title: The Growth Inhibition and Apoptosis-promoting Effect of rSIFN-co to the Cervical Carcinoma Cells in Vitro
Abstract: Objective:To investigate the effect of rSIFN-co,consensus Interferon(Infergen),IFNα-2b(Interferon α-2b)and chemotherapeutics on cervical cancer cell line Caski in vitro.Methods:After Caski cervical cancer cells were cultured in vitro with rSIFN-co、Infergen、IFNα-2b and cisplatin(DDP)at the concentration of 0.156 μg/ml、0.625 μg/ml、2.5 μg/ml and 10 μg/ml respectively for 72 hours,cell proliferation was mensurated by MTT assay,cell inhibition rate was calculated.Then after Caski cervical cancer cells were treated with rSIFN-co、Infergen、IFNα-2b and DDP at the concentration of 0.156 μg/ml、0.625 μg/ml、2.5 μg/ml for 72 hours,cell apoptosis was determined by flow cytometry.Results:rSIFN-co、Infergen、IFNα-2b and DDP could induce inhibition and apoptosis of Caski cells.The inhibition and apoptosis of Caski cells which had been induced by rSIFN-co and DDP were better than which had been induced by Infergen and IFNα-2b at the same concentration,and DDP was more better than rSIFN-co.Conclusions:rSIFN-co has antineoplastic effect by inhibiting proliferation and inducing apoptosis in Caski cervical cancer cell in vitro.The effect is inferior to DDP,but better than the same type interferon and general type I interferon.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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