Title: Experimental study on Yisheng injection for a novel accelerated rat cardiac graft failure model induced by ischemia/reperfusion injury
Abstract: Objective: To investigate whether the traditional chinese medicine Yisheng injection can reduce the progress of a novel accelerated rat cardiac graft failure model induced by ischemia/reperfusion injury.Methods: 40 female closed colony SD rats were divided into two groups at random.In the control group,the isolated rat hearts were preserved in 0.9% sodium chlorine solution at 4℃ for 3 hours before transplantation,while in the experimental group,the hearts were preserved in 0.9% sodium chlorine solution containing Yisheng injection 0.5 mg/ml at 4℃ for 3 hours before transplantation.After transplantation,the experimental group recipients were treated with Yisheng injection 8 mg/(kg·d) for 9 days.The specimens of blood were harvested from recipients before transplantation and on 1,3,5,7,9 day after transplantation.The changes of MDA and SOD were observed in two groups.Grafts were excised at 12 weeks after transplantation to assess the graft vasculopathy and interstitial fibrosis using HE and Masson staining for two groups.Results:The MDA and SOD of two groups were similar before transplantation.However,the MDA of the two groups increased and the SOD of two groups decreased,especially the first day after transplantation,but it was more significant in the control group.Then,The MDA and SOD of the two groups recovered to normal gradually,but the experimental group recovered to normal on the 5 day after transplantation,faster than the control group,which recovered to normal on the 9 day after transplantation.By 12 weeks,compare the changes of histology of the grafts of the two groups,grafts vasculopathy and interstitial fibrosis were decreased significantly in experimental group.Conclusion: It demonstrated that Yisheng injection can decrease ischemia/reperfusion injury and reduce the development of rat cardiac chronic graft failure in a novel accelerated rat cardiac graft failure model induced by ischemia/reperfusion injury.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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