Title: Cloning,Expression and Insecticidal Activity of cry8Ea2 Gene from Bacillus thuringiensis Strain B-DLL
Abstract:The full length cry8Ea gene was amplified by PCR using a pair of special primers,JJX5 and JJX3,designed according to cry8-type genes sequences,and inserted into E.coli expression vector pET-21b to obt...The full length cry8Ea gene was amplified by PCR using a pair of special primers,JJX5 and JJX3,designed according to cry8-type genes sequences,and inserted into E.coli expression vector pET-21b to obtain the recombinant plasmid.Nucleic acid sequence analysis showed that this gene was 3 495 base pairs encoding 1 164 amino acids,which were homolog of 99.31% compared with Cry8Ea1,the molecular weight of the protein was 131.8 kDa with isoelectric point pH4.71.This gene sequence had been registered in GenBank(accession number was EU047597),and named cry8Ea2 as a novel gene by Bacillus thuringiensis Delta Endotoxin Nomenclature Committee.The result of SDS-PAGE indicated that cry8Ea2 gene could be expressed as a 130 kDa protein in E.coli BL21(DE3) strain induced by IPTG.Bioassay of the expression product from the cry8Ea2 gene showed evident toxic to the larvae of Popillia flavosellata,Holotrichia parallela and Plagiodera versicolora,with the corrected mortality of 46.67%,55.56% and 33.33%,respectively.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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Cited By Count: 1
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