Title: IL-25 induces expression of Th2 inflammatory factor by nuocyte in asthma of mouse
Abstract:Objective To study the effect of Interleukin-25 on expressing Th2 inflammatory factor in mouse asthma by nuocyte cells. Methods Nuocytes were collected from mouse spleen of OVA-induced asthma model by...Objective To study the effect of Interleukin-25 on expressing Th2 inflammatory factor in mouse asthma by nuocyte cells. Methods Nuocytes were collected from mouse spleen of OVA-induced asthma model by density gradient centrifugation. Samples were divided into 3 groups according to different intervention conditions:Interleukin-25 group; control group; anti-Interleukin-25 group. The expression of Interleukin-5 and Interleukin-13 proteins in nuocyte cells supernatant was detected by ELISA. The expression of Interleukin-5 and Interleukin-13 in nuocyte cells was detected by Western blot. The expression of Interleukin-5 and Interleukin-13 mRNA was detected by RT-PCR. The number of nuocyte cells was detected by flow cytometry. Results The expression of Interleukin-5 and Interleukin-13 protein and related gene in Interleukin-25 group was higer than that in the control group or anti-Interleukin-25 group( P 0. 05). The number of nuocyte cells in Interleukin-25 groupwas more than that in control group or anti-interleukin-25( P 0. 05). Conclusions Interleukin-25 can promote the expressing Interleukin-5 and Interleukin-13 in asthma by nuocyte cells and promote the occurrence and development of asthma.Read More
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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Cited By Count: 1
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