Title: Selection of Optimal Laser Dosage of 510.6 nm Laser Irradiation Inducing Apoptosis of Rabbit Vascular Smooth Muscle Cells
Abstract: Objective To select an optimal laser dosage of 510 6 nm laser irradiation which could induce apoptosis of vascular smooth muscle cells (VSMC). Methods The rabbit VSMC cultured in vitro were irradiated by 510 6 nm copper vapor laser with a power density of 100, 200 or 300 mW/cm 2 and an energy density of 100, 150, 200, 210, 300 J/cm 2, respectively. TUNEL staining was used to label apoptotic cells of VSMC irradiated at different dosages. Results Apoptotic rate in all laser irradiation groups was 2 9 to 14 7 times higher than that of control group(P0 05). The apoptotic rate of VSMC irradiated at 300 mW/cm 2 for 667 s was 30%. Conclusion Apoptotic rate of rabbit VSMC irradiated at 300 mW/cm 2 for 667 s by 510 6 nm copper vapor laser was relatively higher.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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