Title: Comparison of Three Methods for Determination of Blood Cholinesterase Activity
Abstract: Objective] To compare three methods for measuring blood cholinesterase (ChE) activity. [Method] Blood ChE activity was determined in 132 samples taken from 74 healthy adults non exposed to ChE inhibitor insecticides and 20 intoxicated patients by three different methods. Method A was the improved Ellman spectrophotometry adequate for measuring whole blood,erythrocyte and plasma ChE. Method B was used for measuring erythrocyte acetylcholinesterase (AChE) by a spectrophotometric field kit (Test Mate OP). Method C was used acetylbromocholine as the substrate for measuring whole blood ChE by a test paper. [Results] The reference values of blood ChE activity were established after measuring the blood samples from subjects non exposed to insecticide by three methods. The blood ChE activity was inhibited in 18 cases of organophosphate or its mixed insecticides intoxication and in one case of carbamate intoxication by any method. The activities of erythrocyte AChE,whole blood and plasma ChE measured by method A,and whole blood ChE measured by method C were all significantly correlated with the activities of erythrocyte AChE measured by method B ( r =0 928,0 939,0 638 and 0 906, P 0 01). [Conclusion] All three methods can be used for measuring inhibition of blood ChE activity in patients of organophosphate intoxication. Each has its own strong and weak points.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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