Title: Effect in vivo of norcantharidin on proliferation and growth of implanted tumors of human gallbladder carcinoma in nude mice
Abstract: AIM: To explore the effect in vivo of norcantharidin (NCTD) on proliferation and growth of implanted tumors of human gallbladder carcinoma in nude mice. METHODS: Animal model simulating human gallbladder carcinoma was created by subcutaneous implantation of cultured GBC-SD cell lines into nude mice. Mice used in experiments were randomly divided into four groups and were taken different treatments. Tumor dimensions, tumor inhibitory rates of each group were respectively evaluated. Flow cytometry was used to measure the cell cycle and the induction of apoptosis. Immunohistochemistry was taken to determine the expressions of PCNA, Ki-67, cyclin Dl and p27 gene proteins in each paraffin section of every group. RESULTS:LD50 of NCTD was 139.96 mg·kg-1. Volume of implanted tumors of NCTD group vs control group [ (5.6 ± s 0.4) cm3 vs (9.8 ± 0.6) cm3, P 0.01] in nude mice after treatment showed the former was smaller than that of the control group, but with increase of tumor inhibitory rate (43 f33 8) %. The flow cytometric profiles in NCTD group and NCTD+FU group revealed decrease percentage of implanted tumor cells in the S phase,simul taneously with the increase rate of cell apoptosis. The expressions of PCNA, Ki-67, cyclin D1 were significantly decreased together with significant increase of expression p27, in paraffin sections of NCTD group. CONCLUSION: NCTD can inhibit the proliferation and growth of human cholecystic carcinoma cells in implanted tumor of nude mice and further intensified effect could be achieved with combination of FU. Its anti-tumor mechanism might be correlated with inhibition of cell proliferation,blockage of cell cycle,induction of cell apoptosis,alteration oi the expression of proliferation and cycle control-related gene proteins such as PCNA,Ki-67 ,cyclim Dl ,p27.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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Cited By Count: 1
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