Title: Modified focal ischemic model of rat middle cerebral artery occlusion using nylon suture embolization
Abstract: Objective To determine modified method for rat focal ischemia model with more stable infarct volume. Methods The middle cerebral arteries were embolized with nylon suture coated with liquid silicon glue in 24 rats. After 1, 2, 6 and 24 hours ischemia and 24 hours reperfusion, the regional cerebral blood flow was monitored and infarct volume and extent of brain edema were determined. Results After ischemia, the regional cerebral blood flow reduced below 25% of baseline in all rats. TTC stain revealed infarction or ischemia in cortex and caudaputaman of occluded sides in all rats. There is a significant difference between 1-hour ischemia group and other groups. No significant difference was found between 2, 6 and 24 hours groups. There is no significant difference in edema extent among each group. Conclusion Combined with regional cerebral blood flow monitoring, liquid silicon glue coated nylon suture embolization for more than 2 hours can produce stable infarct volume rat focal ischemia model.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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