Title: Optimization of fermentation medium of chitinase-producing bcterium C4 and its synergistic effect to Bacillus thuringiensis
Abstract: To improve the chitinase activity of chitinase-producing bcterium C4,a optimal medium was acquired after medium optimization.The optimal conditions were as follows: peptone 0.8%,yeast extract 0.8%,KNO3 0.8%,MgSO4 0.05%,KH2PO4 0.03%,pH 7.5.Adding 1% colloid chitin to the medium to induce the bacterium C4 to produce chitinase.Results showed that: the fermentation period of C4 shortened to 4d and the chitinase activity boost to 5.95 U/mL.Using the mixture of Bt and C4 fermentation broth at the ratio of 1 to 1 to feed silkworm,the mortality rate of Bt bove 47.8% that of using Bt alone.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot