Title: The Influence of Ulinastatin on TNF-α,MDA,PMN during Cardio-pulmonary Bypass in Patients undergoing Heart Valve Replacement
Abstract: Objective To observe the protective role of Ulinastatin in the patients with lung injury during perioperation of Heart valve replacement when undergoing Cardiopulmonary bypass.Methods 30 patients undergoing valve replacement were randomly allocated into Ulinastatin group and control group(15 cases in each group).In Ulinastatin group,6000 IU·kg-1 of Ulinastatin were respectively injected before CPB and added in priming solution to accompanoy turnging point into vivo.In control group 0.9% NaC1 solution was as a placebo.Serial blood samples were collected at the following intervals respectively:After anesthesia induction(T1),45min after CPB(T2),5min before the end of CPB(T3) and 6h(T4),and 24h after the end of CPB(T5).We measured the serum levels of TNF-α by Elisa method at the time T1~T5 and the malondialdehyde(MDA) by thiobarbituricacid chromatometry,and detected the polymorphonuclear leucocyte in the blood from T1 to T3.Results The serum levels of TNF-α,MDA and PMN gradually increased with time and reached the peak at 5min beforethe end of CPB.And the TNF-α still maintained a high level at 24h after the end of CPB.The serum levels of TNF-α in Ulinastatin group was significantly lower than that in control group(P0.05) at the time T3~T5.The serum levels of MDA and the PMN in Ulinastatin group was significantly lower than that in control group(P0.05) at the time T2-T3.Conclusion Ulinastatin could reduce the release of TNF-α,and lipid peroxidation(MDA) and the PMN,so it can protect the injury of lung.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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