Title: SSR analysis on genetic structure of Chinese jujube in North Shaanxi
Abstract: 【Objective】The purpose of this study was to elucidate the genetic structure of Chinese jujube at molecular level,and reveal the relationship between genetic differentiation and cultivars.【Method】Using 7pairs of SSR primers,54germplasms from 4geographical regions and 8cultivar groups along the Yellow River in North Shaanxi were selected to assess their genetic structure.【Result】The mean observed heterozygosity of the 4regions(Ho,0.909)was higher than the mean expected heterozygosity(He,0.634)with the mean Shannon's information index(I)of 1.141and negative mean fixation index and inbreeding coefficient.The highest effective number of alleles(Ne),Iand He were found in Jiaxian while the lowest were found in Yanchuan.Goutouzao had the lowest in Ne,I,and He among all cultivars.The geneticvariation in variety group(91%)was less than that in geographic region(97%),and they both were much higher than differentiation among groups(3% and 9%).Genetic differentiation coefficient of cultivar groups(0.136)was greater than that of geographical regions(0.042),while mean gene flow among cultivar groups(1.584)was less than that among geographical regions(5.653).When the Nei's genetic distance was 0.13,the 4geographical regions were clustered into 2groups:Qingjian-Mizhi-Jianxian group and Yanchuan group.When the Nei's genetic distance was 0.99,8cultivar groups UPGMA were divided into 2clusters:Tuanzao-Muzao group and Gouzao group.Based on neighbor-joining method,54germplasms were clustered into 2groups.Tuanzao varieties from Yanchuan,Qingjian and Mizhi were in one group while all varieties from Jianxian were in one group,highlighting a close affinity among cultivar groups while Goutouzao was distantly related with other cultivars.【Conclusion】Jujubes planted in North Shaanxi had moderate heterozygosity,high genetic diversity,high variability,and low inbreeding degree.With considerable gene flow and narrow differentiation among geographical regions,multivariate genetic relationships among accessions were not correlated with geographic regions,but were closely correlated with cultivar groups.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 1
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