Title: Purification of Tuckahoe Polysaccharide and Determination of Its Molecular Weight
Abstract: Tuckahoe polysaccharide was extracted by boiling water and precipitated by alcohol,and purified using ion-exchange column chromatography,and its molecular weight was determined by high performance gel filtration chromatography(HPGFC).The results showed that Sevag method was effective for removing proteins from crude polysaccharide to obtain refined tuckahoe polysaccharide(TAP);two components,neutral polysaccharide TAP1 and acidic polysaccharide TAP2,were got through DEAE-650C column chromatography purification,whose molecular weight were determined by HPGFC as 11 721 and 44 065,respectively.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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