Title: Simultaneous and rapid detection of influenza virus A and B by fluorescent quantitative real-time PCR.
Abstract: Objective To develop a method of rapid detection of influenza virus A and B by multiplex real-time quantitative RT-PCR. Methods By the M gene of influenza virus A and HA gene of influenza virus B, a set of primers and Taqman probe were designed, respectively. After the real-time assay system was established, the sensitivity and the quantitative curve of the multiplex assay were determined using tenfold serial dilution of TCID_ 50 . Results For the influenza virus A and B, the sensitivity of the assay were 2.56×10~ -5 and 5.0×10~ -5 TCID_ 50 and the regression coefficient of the quantitative curve were 0.997 and 0.998, respectively. The results suggested there was no interference between the primers and probes of influenza virus A and B. Conclusion The detection system based on multiplex real-time RT-PCR was rapid, sensitive and steady, by which the viral load could be detected also.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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