Title: Cloning and Prokaryotic Expression of the ORF1 and the ORF2 Truncated Genes of Porcine Circovirus Type 2
Abstract: PCV2ORF1 and ORF2truncated genes were amplified by PCR from the double-copies of infectious DNA clone and inserted into the prokaryotic expression vector pQE30 used double restriction-enzyme Kpn I and Hand III digestion respectively.The recombinants were constructed successfully used PCR、double restriction-enzyme digestion and sequenced.Purpose proteins were detected expression by SDS-PAGE and Western-blot after induced by IPTG.The results suggest an foundation for protein function and Mcab research.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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