Title: HPLC-ECD determination of flavonols from Ginkgo biloba leaf extracts in rat plasma and its pharmacokinetic study
Abstract: Objective:To establish a sensitive method for the quantification of flavonols from rat plasma after intragastric administration of Ginkgo biloba leaf extracts.Methods:HPLC with electrochemical detection(ECD) was employed to quantify quercetin,isorhamnetin and kaempferol simultaneously after plasma samples were hydrolyzed by glucuronidase.The sample was separated by Agilent Zorbax Eclipse XDB-C8(150 mm×4.6 mm,5 μm) column and eluted by the mobile phase composed of methanol-isopropanol-phosphate buffer(NaH2PO4-H3PO4,pH=2)(15∶15∶70) at column temperature of 30 ℃,and the flow rate was 0.8 mL·min-1.The potential of analysis cell was selected as 200 mV.Results:The analysis of plasma samples could be performed in 20 min with the quantitative limit of 0.5 ng·mL-1 and average recovery of 88.7%,86.2% and 87.0% for quercetin,isorhamnetin and kaempferol,respectively.The intra-and inter-day precision(RSD) was measured less than 6.6% and 14.1%,meanwhile the accuracy was calculated as 92.7%-105.3%.The concentration-time data after oral administration of 10 mg·kg-1 Ginkgo biloba leaf extracts showed that flavanols was absorbed rapidly and presenting the fist peak appeared at 0.17-0.23 h and the second peak at 6 h.The elimination half-life time(t1/2k) was calculated as(3.53±1.88) h,(6.94±4.05) h and(3.97±2.30) h for quercetin,isorhamnetin and kaempferol,respectively.Conclusion:The method developed in the present study using HPLC-ECD is highly sensitive,accurate,rapid and suitable for pharmacokinetic study of Ginkgo biloba leaf extracts in rats.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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