Title: ROLE OF APOPTOSIS IN RADIATION-INDUCED MOUSE SPLEEN DAMAGE AND REPAIR AND ITS MECHANISM
Abstract: To
investigate the role of apoptosis in radiation-induced damage and repair of mouse spleen and
provide the basis for expounding the corresponding mechanism as well as for the prevention
and treatment of acute radiation. Using in situ terminal labeling (ISTL), DNA electrophoresis and
immunohistochemical techniques, we studied the dynamic changes of apoptosis of mouse
spleen lymphocytes and the expression of bax and bcl-2 gene products after 2, 4, 6 and 8Gy
whole body γ-irradiation. Results (1) At the early stage after irradiati on the percentages of
lymphocyte apoptosis increased rapidly, for instance, 4h and day 1 after 6Gy γ-irradiation the
apoptotic lymphocyte were 4.9 and 9.7 times those of control values; At the same time, with the
increase of radiation doses the percentages of lymphocyte apoptosis increased rapidly, for
instance, 8h after 2, 4, 6 and 8 Gy γ-irradiation the apoptotic lymphocyte were 3.7, 5.4, 6.2 and
8.3 times those of control values respectively. (2) After 6 Gy γ-irradiation typical morphological
characteristics of spleen apoptotic lymphocyte in early, middle and late stages were observed
by transmission electron microscope. Using DNA gel electrophoresis technique the spleen
lymphocytes displayed characteristic DNA ladders 4 and 6 h after 6 Gy γ-irradiation.The
percentage of lymphocyte apoptosis measured by ISTL was 3.4 times higher than that of control
value 4 h after 6 Gy γ-irradiation. (3) Abnormal expression of bcl-2 and bax gene products in
irradiated lymphocytes confirmed that they played a vital role in the apoptosis of mouse spleen
lymphocytes.Conclusion Apoptosis played an important role in the whole process of
radiation-induced damage and repair of mouse spleen lymphocyte and Bcl-2 and Bax proteins
could regulate the process of lymphocyte apoptosis.
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
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