Title: Expression and Cloning of a Multiple Stress Responsive Gene(OsMsr3) in Rice
Abstract: To understand mechanism(s) underlying stress responses in rice and to discover new stress-tolerance genes,we conducted a global genome expression profiling of the indica cultivar Pei'ai 64S subjected to heat,drought and cold stresses.Expression profiles were obtained for leaf and panicle tissues at different development stages of plants under the stress conditions using the GeneChip Rice Genome Array(Affymetrix) representing 51279 transcripts from both japonica and indica rice plants.We identified a multiple stress responsive gene OsMsr3(Oryza sativa L.multiple stresses responsive gene3,GenBank accession:FJ383169),which was highly induced in both leaf and panicle,in response to heat,drought and cold stresses.The expression profile of OsMsr3 obtained by the microarray analysis was further confirmed by quantitative real-time RT-PCR analysis of the gene.The two sets of data are very well matched,suggesting that OsMsr3 is really a multiple stress responsive gene in rice.In order to study its function in stress tolerance,we cloned the cDNA of the gene through amplification by RT-PCR.Sequence analysis showed that the cDNA consists of an open reading frame of 480 bp,encoding a protein of 160 amino acid residues with M.W.≈18.0 kD and pI≈6.8.The alignment of multiple amino acid sequences revealed the exis-tence of a conserved HSP20 sequence domain at the C-terminus.Similarity analysis showed that the rice sHSP shares 33.7%-97.5% identities with sHSP sequences found in other plants.Analysis of the putative promoter region identified 6 matches to cis-elements related to stress responses using PlantCARE software(http:∥bioinformatics.psb.ugent.be/webtools/plantcare/html/).Based on the results obtained,we propose that OsMsr3 might play an important role in rice stress responses.Further study on the function of the gene is in progress.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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