Title: A method for Detecting the Binding Activities of Aminopeptidase N Inhibitors to Tumor Cell by Fluorescein
Abstract:Objective: To build a fluorescein labeling method to detect the bingding of aminopeptidase N inhibitors with tumor cells. Methods: Aminopeptidase N inhibitors LYRM03 and Bestatin were labeled with flu...Objective: To build a fluorescein labeling method to detect the bingding of aminopeptidase N inhibitors with tumor cells. Methods: Aminopeptidase N inhibitors LYRM03 and Bestatin were labeled with fluorescein isothiocyanate(FITC), and fluorescein-labeled compounds of FITC-LYRM03 and FITC-Bestatin were purified. The relationship between binding activities to tumor cells and inhibitory activities of aminopeptidase N inhibitors was detected by using fluorescence microscope and flow cytometer. Results: Compounds of LYRM03 and Bestatin had inhibitory activities to aminopeptidase N of tumor cells. Fluorescein-labeled FITC-LYRM03 and FITC-Bestatin had different binding activities to tumor cells. Conclusion: There was consistency between binding activities of tumor cells and inhibitory activities of aminopeptidase N inhibitors.Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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