Title: A Comparative Study of Abnormalities of Cells in Roots of Vicia faba Induced by 8-Hydroxylquinoline and Hydroquinone
Abstract:Root tip of vicia faba,first treated with 8-HQ and HQ separately and then cultivated for recovery,are sliced and observed according to Feulgen staining method with material treated at low temperature ...Root tip of vicia faba,first treated with 8-HQ and HQ separately and then cultivated for recovery,are sliced and observed according to Feulgen staining method with material treated at low temperature as the negative control.The micronucleus rate of cells in the Roots tips treated with the two chemicals each of different densities and the effect of the chemicals on mitosis index and chromosome aberration are examined.After comparing with the control sample the following conclusions are drawn:(1) Both mutagens can induce high frequency micronucleus;8-HQ can also produce binuclear cells at a relatively high frequency.(2) HQ of both different densities can obviously increase mitosis index and effectively accumulate mid-term split phase while 8-HQ exerts litter effect on the increase of mitosis index but shows remarkable restraining effect at 0.004 mol·L-1.(3) Both mutagens can induce chromosome aberration and nuclear aberration of other kinds.The results of this study show that both 8-HQ and HQ are effective cell board restraining agents and strong mutagens inducing aberration in the number and structure of chromosomes.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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