Title: L-type calcium channel participates in the protective effect of emulsified isoflurane on the primary cardiac myocytes hypoxia/reoxygenation injury in neonatal rats
Abstract: Aim To discuss the mechanism of protective effect of emulsified isoflurane(EI) on neonatal rat hypoxia/reoxygenation(H/R) cardiac myocytes of primary culture and the association with reduction of intracellular calcium overload.Methods The H/R injury model on the basis of in vitro neonatal rat cardiac myocytes culture was prepared and divided into 5 groups randomly,namely,normal control group(N group),H/R group,H/R+fat emulsion group(F group),H/R+1.68 mmol·L-1 EI group(EI group),H/R+1.68 mmol·L-1 EI +10 umol·L-1 Bay K8644 group(EIB group).The clean liquid on the cellular culture was extracted in each group to mensurate LDH and cellular homogenate was made to mensurate SOD and MDA;the microscope was placed upside down to observe the characteristics of cardiac myocytes growth and changes of its forms;the calcium probe Fura-2 AM was and dyed the flow cytometry was used to inspect the calcium concentration in cardiac myocytes.Results Compared to N group,the LDH,MDA,cTnI and intracelluar Ca2+ concentration of other groups all increased(P0.05) and SOD declined(P0.05).Compared to H/R group,SOD of F group declined(P0.05),and the differences of the LDH,MDA,cTnI and intracelluar Ca2+ concentration had no statistic significance(P0.05);LDH,MDA,cTnI and intracelluar Ca2+ concentration of EI and EIB group declined(P0.05) and SOD increased(P0.05).Compared to F group,the LDH,MDA,cTnI and intracelluar Ca2+ concentration of EI group declined(P0.05) and SOD increased(P0.05).Compared to EI group,the LDH,MDA,cTnI and intracelluar Ca2+ concentration of EIB group increased(P0.05) and SOD declined(P0.05).Conclusions EI has the protective effect on the neonatal rat cardiac myocytes with H/R injury,and its mechanism may be associated with blocking L-type calcium channel and reduction of intracellular calcium overload.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot