Title: Effect of lysophosphatidic acid on metastasis and expression of various metastatic molecules of transplanted ovarian cancer in nude mice
Abstract: Objective:To investigate the effect of lysophosphatidic acid(LPA)on metastasis and expression of various meta- static molecules of ovarian cancer in nude mice and discuss the mechanism for its metastasis-stimulating effects.Methods:Twenty nude mice were randomly divided into two groups,control group and LPA group.In LPA group SKOV3 cells were pretreated with LPA and 6×10~6 SKOV3 cells were transplantated intraperitoneally to nude mice.In control group the same quantities of SKOV3 cells not pretreated with LPA were transplantated intraperitoneally to nude mice.When every nude mouse naturally died,the size and weight of the transplantated tumor and the number of the metastatic lesions were observed and recorded.Ex- pression of vascular endothelial growth factor(VEGF),matrix metallproteinase-2(MMP2)and cyclo-oxgenase 2(COX-2)in transplantated tumors were determined by immunohistochemistry and flow cytometry.Results:The rate of tumor formation was 100% in both LPA group and control group.But the growth rate and weight of the transplantated tumors and the number of the metastatic lesions in LPA group were significantly higher than that of control group(P<0.05).Expression of VEGF,MMP2, and COX-2 increased significantly in LPA group compared with control group(P<0.01).Conclusion:LPA simulates growth and invasive metastasis of ovarian cancer cells in the peritoneal cavity.The effects of LPA may be related with stimulating the expres- sion of various metastatic molecules in tumor tissues.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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