Title: Quantitative analysis of minimal residual disease cells DNA in children with acute lymphoblastic leukemia
Abstract: Objective
To explore the clinical significance of quantitative analysis of minimal residual disease (MRD)
cells DNA in children with acute lymphoblastic leukemia (ALL). Methods By using the
competitive polymerase chain reaction (CPCR) technique which was based on the 5terminal
modified primers to obtain the internal standard template, the Tcell receptor (TCR)V2D3 gene
rearrangements were detected in 162 bone morrow samples collected from 70 patients with
ALL, and MRDDNA were analysed quantitatively in patients with complete remission (CR).
Results (1) The frequencies of TCR V2D3 rearrangements were 81% in ALL, 83% in BALL and 1/4
in TALL, respectively. (2)MRDDNA was detected in 92 samples collected from 48 patients with
CR, and the MRD showed a descending tendency year by year in the patients, but not the
patients with CR less than 3 months. The highest MRD DNA level was found in the patients with
CR of 412 months and showed a positive correlation with the relapse of ALL. MRDDNA was
elevated (0.05%) 7 months prior to the relapse. The patients with continuous complete
remission (CCR) over 3 years and with negative MRD or MRD0.05% persistently could be
considered to terminate the chemotherapy. Conclusion The CPCR method was a simple,
sensitive and accurate MRDDNA quantitative method. It may play an important role in
evaluating the chemotherapy efficiency, predicting relapse and prognosis and guiding clinical
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
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