Title: The effect of LPS on the expression of CD14 and TLR4 in rat Kupffer cells
Abstract:Objective To explore the effect of LPS on the expression of CD14 and Toll like receptor 4 (TLR4) in rat Kupffer cells (KCs).Methods In the rat KCs stimulated with LPS,the expression of CD14,TLR4,TNFα ...Objective To explore the effect of LPS on the expression of CD14 and Toll like receptor 4 (TLR4) in rat Kupffer cells (KCs).Methods In the rat KCs stimulated with LPS,the expression of CD14,TLR4,TNFα and IL 6 was detected.Results LPS could obviously up regulated the expression of CD14 and TLR4 in a dose dependent manner (LPS 10?mg/L,CD14/GAPDH=0.73,TLR4/GAPDH=0.63) and time dependent manner (0.5 24.0?h,the expression of CD14 reached the peak during 3 to 6 h,CD14/GAPDH=0.86).The expression of CD14 and TLR4 in KCs stimulated by the active mediators from KCs which had been exposed to LPS 1 h was obviously increased.Conclusion There were a close correlation between LPS or the active mediators from KCs induced by LPS and the expression of CD14,TLR 4.It is implied that the increases of TLR4 and CD14 expression may be induced by LPS in 1 3?h,and later further increases of TLR4 and CD14 expression may be correlated with the cytokines produced by KCs.Read More
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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