Abstract:Objective:A comparison study of polymerase chain reaction (PCR) and serological typing method was used to detect HLA B27.Methods:78 samples from patients suspected with ankylosing spondylitis (AS) wer...Objective:A comparison study of polymerase chain reaction (PCR) and serological typing method was used to detect HLA B27.Methods:78 samples from patients suspected with ankylosing spondylitis (AS) were genotyped by PCR and some of them were assayed by serological typing.Results:24/36 samples with HLA B27 gene were compared with serological typing ,the results of 5 samples serological typing were dubious or contradictory with PCR genotyping.21/42 samples without HLA B27 gene were compared with serological typing,the results of 3 samples serological typing were dubious.Conclusion:The method of B27 genotyping by PCR is more rapid,inexpensive and exact than phenotyping by serology.Read More
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
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