Title: Protection of TGF-β1 against intestinal mucosal ischemia-reperfusion injury in rats
Abstract: Aim:To investigate protection of transforming growth factor-β1(TGF-β1)against intestinal mucosal ischemia-reperfusion(I/R)injury in rats.Methods:Thirty SD rats were allocated into 3 groups(10 rats in each group)at random:sham-operation group(group A),I/R group(group B)and experimental group(group C).Superior mesenteric artery(SMA)in group C was blocked for 60 min,then reperfused for 120 min,and administered with TGF-β1 through SMA 10 min before blockage.In group B,animals underwent the same procedure as group B but taking normal saline instead of TGF-β1.In group A,SMA was only isolated but not blocked,the other procedures was the same as group B.Intestinal mucosal damage was detected by HE staining and graded according to Chiu's scoring system.D-lacate in portal vein plasma was also detected.Results:There was more destruction of intestinal villous in group B than that in group A,but the villous histology was improved in group C.The differences of Chiu's score and level of D-lacate in portal vein plasma were significant among 3 groups(F=20.490,33.084,P0.001).There was a linear relationship between intestinal histological damage and portal vein plasma D-lactate level(r=0.881,P0.001).Conclusion:TGF-β1 exerts a protective effect on functional and morphological injury induced by I/R,which results in an increased intestinal permeability.Plasma D-lactate level indirectly mirrors permeability of the intestinal mucosa and can be used to access the grade of intestinal mucosal damage.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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