Title: Development of a new-type reverse transcription-multiplex nested polymerase chain reaction for detection and differentiation of wild-type and c-strain vaccine viruses of classical swine fever virus
Abstract:Twenty five strains classical swine fever virus(CSFV)genomic sequences including wild-type and vaccine virus pubulished in GenBank were aligned,and a pair of primers corresponding to the highly conser...Twenty five strains classical swine fever virus(CSFV)genomic sequences including wild-type and vaccine virus pubulished in GenBank were aligned,and a pair of primers corresponding to the highly conserved regions of CSFV genome was used as a common primer pair in the first round of the RT-nPCR,amplified fragment length 609 bp.Another primers specific for CSFV vaccine virus,respectively,were used for the second round of the RT-nPCR together with the forward common reverse primer,amplified fragment length 237 bp,in order to develop a reverse transcription-multiplex nested polymerse chain reaction for differentiation wild-type and C-strain vaccine virus with the characteristic of sensitivity and specificity.It can completely differentiate wild-type of different subgroups from vaccine virus,which has no specific reaction with bovine viral diarrhea virus and other virus of pigs.This assay can mark a detection,accurately and rapidly,for CSFV,which can prevent it from extending.Besidely,it can differentiate pigs infected with wild-type CSFV from those vaccinated with vaccine virus,which can minimizes the possibility of miss-killed vaccinates during outbreaks.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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