Title: Research in anti-tumor activity of the novel GSK-3β inhibitor-polypyridyl ruthenium complexes
Abstract:Aim To investigate the mechanism of apoptosis on human glioma SWO-38 cells induced by polypyridyl ruthenium complex∧-[Ru(bpy) 2(pyip) ]2+as the inhibitor of GSK-3βin vitro.MethodsThe right complex∧-[R...Aim To investigate the mechanism of apoptosis on human glioma SWO-38 cells induced by polypyridyl ruthenium complex∧-[Ru(bpy) 2(pyip) ]2+as the inhibitor of GSK-3βin vitro.MethodsThe right complex∧-[Ru(bpy) 2(pyip) ]2+with highest activity and lowest toxicity from eight kinds of polypyridyl ruthenium complexes with chiral separation were filtered by MTT colorimetric assay,and the proliferation of the cells with different concentrations of the complex was detected;FITC-AnnexinⅤ/PI double labelling was used to detect the apoptosis of SWO-38 cells;Western blot was employed to analyze the expression of p-GSK-3β Ser9 protein.Results MTT showed the complex could inhibit the growth of SWO-38 cells obviously;compared the dosing concentrations10 μmol·L -1 and that of the control group,the proliferation rates of SWO-38 cells had some differences(P 0.05) ;Flow cytometry showed the complex could significantly induce the apoptosis of SWO-38 cells;Western blot showed that compared with the control group,the level of p-GSK-3β Ser9 was up-regulated after treatment with 10,30,50μmol·L-1 concentrations of the complex(P0.05) .Conclusions The anti-tumor mechanism of the ruthenium complex∧-[Ru(bpy) 2(pyip) ]2+may correlate with the inhibition of the activity of GSK-3βprotein which results in the apoptosis of SWO-38 cells,and these effects were dose dependent.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot