Title: DOSE RESPONSE CURVE FOR MICRONUCLEUS OF CYTOKINESIS-BLOCK METHOD IN HUMAN LYMPHOCYTES AFTER ~(60)Co-GAMMA RAY EXPOSURE
Abstract: The micronucleus technique of cytokinesis block has been proposed as a new method to measure chromosomeal damage in cytogenetics. The cytokinesis is blocked by using cytochalasin B (Cyt-B), and micronuclei are scored in cytokinesis-blocked (CB) cells. This can easily be done owing to the appearance of binucleate cells and large numbers accumulated by adding 3. 0 g/ml cytochalasin B at 44 hours and scoring at 72 hours. The results show that the optimum concentration of Cyt-B is 3. 0 g/ml. The Cyt-B itself can not induce the increase of micronuclei. The micronucleus frequency of normal individuals in vivo is 4. 6 + 2. 0 micronucleus/500 CB cells. For lymphocytes irradiated in vitro, there is an approximately linear relationship between the frequency of induced micronuclei and irradiation dose. The formula is Y = 0. 36D + 2. 74 (r2 = 0. 995,P 0. 01) , Because the cytokinesis block method is simple and reliable, it is effective for assaying chromosome damage caused by genetic toxic materials.
Publication Year: 1993
Publication Date: 1993-01-01
Language: en
Type: article
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