Title: Radiosensitivity Evaluation of Human Tumor Cell Lines by Single Cell Gel Electrophoresis
Abstract: Objective To explore the feasibility of determining radiosensitivity of human tumor cell lines in vitro using single cell gel electrophoresis(SCGE).Methods Three human tumor cell lines were selected in this study,HepG2、EC-9706 and MCF-7.The surviving fraction(SF)and DNA damage were detected by MTT assay,nested PCR technique and comet assay respectively.Results MTT assay:The SF of HepG2 and EC-9706 after irradiated by 2、4and 8Gy was lower significantly than that of MCF-7,which showed that the radiosensitivity of HepG2 and EC-9706 was higher than that of MCF-7.But there was no statistical difference of SF between HepG2 and EC-9706.SCGE: The difference of radiosensitivity among these three tumor cell lines was significant after 8Gy γ-ray irradiation.Conclusion The multi-utilization of many biological parameter is hopeful to evaluate the radiosensitivity of tumor cells more objectively and exactly.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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