Title: Effect of 2-Methoxyestradiol on the Proliferation and Apoptosis of Human huh7 Cells in Vitro
Abstract: Objective: To evaluate the effect of 2-methoxyestradiol(2ME2) on the proliferation and apoptosis of human huh7 cells,and to study its molecular mechanisms.Methods: The human huh7 cells were exposed to different concentration of 2-ME2,the methyl thiazolyl tetrazolium(MTT) assay was used to evaluate the cells cytotoxicity;the flow cytometry was adopted to test the cells cycle and apoptosis;Western blot was used to detect the expression of vascular endothelial growth factor(VEGF) and Bcl-2 protein.Results: The growth of huh7 cells was inhibited by 2-ME2 in a time-and dose-dependent manner;2ME2 could inhibit the mitosis of huh7 cells,cells were arrested in G2/M phases,and the huh7 cells in S phase decreased;2ME2 could induce huh7 cells apoptosis,particularly induce cell early apoptosis;Western blot result demonstrated 2ME2 decreased the expression of VEGF and Bcl-2 dose-dependently.Conclusion: 2ME2 can inhibit the proliferation of huh7 cells and induce cells apoptosis,which maybe related to its effect of down-regulating VEGF and Bcl-2.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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