Title: Phenotypical and funcitonal properties of CD4~+CD28~- T cells in Wegener′s granulomatosis
Abstract: Objective To investigate the expression of costimulatory molecules (CosM) CD28/CTLA 4 CD80/CD86,and to analyze the phenotypic and functional features of CD4 +CD28 - T cells in patients with Wegener′s granulomatosis (WG).Methods The expression of CD28,CTLA 4,CD80 and CD86 was measured by multiparameter flow cytometer.CD4 +CD28 - T cells and CD4 +CD28 + T cells from the peripheral blood of WG patients were cultured with different stimuli,and proliferation and cytokine production were assessed.Results CD28 - T cells in WG were increased,which were mainly CD4 + T subset when compared with healthy controls.CD4 +CD28 - T cells expressed high levels of lymphocyte activation markers such as CD25,CD38,CD45RO,HLA DR,as well as CosM ligand CD86.CD4 +CD28 - T cells exhibited increased proliferation response following stimulation with autoantigen proteinase 3 compared to CD4 +CD28 + T cells.Furthermore,anti CD3 stimulated proliferation of CD4 +CD28 - T cells could be enhanced in the presence of antigen presenting cells (APC).However,this proliferation response can not be blocked by CTLA 4 Ig,anti CD80 or anti CD86 monoclonal antibodies.In addition,the levels of IFN γ produced by CD4 +CD28 - T cells were significantly higher than CD4 +CD28 + T cells did.Finally,CD4 +CD28 - T cells were able to act as APC in anti CD3 induced T cell proliferation,this may be due to the cells expressed enhanced levels of CosM ligand CD86 which provide them with APC properties.Conclusion These data indicate that in WG increased CD4 +CD28 - T cells are a subset of in vivo activated autoreactive T cells,which mainly produce Th 1 type cytokine and could act as non professional APC.The activation and proliferation of CD4 +CD28 - T cells are independent of CD28/CTLA 4 CD80/CD86 pathway.
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot