Title: Effects of aspirin and heparin on platelets activation and CD40L expression
Abstract:Objective To explore the relationship between platelets activation and CD40L expression, and to determine whether these biochemical processes of platelets can be influenced by aspirin and heparin. Met...Objective To explore the relationship between platelets activation and CD40L expression, and to determine whether these biochemical processes of platelets can be influenced by aspirin and heparin. Methods Platelets were isolated from healthy volunteers and induced in vitro by various concentrations of ADP and thrombin. Levels of P-selectin (an indicator of platelets activation) and CD40L(an inflammatory marker) expression and their change processes with prolonged inducing time were assessed by flow cytometry. Effects of aspirin, unfractionated heparin (UFH) and low-molecular-weight heparin (LMWH) on platelets activation and CD40L expression were also investigated. Results Both ADP and thrombin dose-dependently enhanced the expression of P-selectin and CD40L of platelets. The levels of P-selectin and CD40L were significantly positively correlated with inducing time extending (P0.05). Aspirin (2.5 μg/ml) had no any effects on platelets activation and CD40L expression induced by both ADP (4 μmol/L)and thrombin (1U/ml). UFH (2.5U/ml) and LMWH (2.5U/ml), either single used or combined with aspirin, very significantly inhibited platelets activation and CD40L expression induced by thrombin (P0.001), but had no effects on platelets induced by ADP (P0.05). Conclusion These results demonstrated that CD40L, an inflammatory mediator, can be expressed on the surface of activated platelets. Aspirin and heparin can not completely inhibit platelets activation and CD40L expression under the condition of various inducers.Read More
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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