Title: Sreening of Apoptosis-related Proteins Affected by Acupuncture in Cerebral Ischemia Reperfusion Injury Rats with Protein Chip Technology
Abstract: Objective To screen the apoptosis-related protein affected by acupuncture in cerebral ischemia reperfusion injury( CIRI) rats using the technology of protein chip. Methods 40 healthy SD rats were randomly divided into four groups,sham operation group,model group,acupuncture points control group( located 0. 3 cm from the left side of acupoints,which are not meridian and acupoints),acupuncture group( Dazhui,Baihui,Renzhong are acupunctured),with 10rats in each group. We extracted ischemic brain tissue after 6 times treatments,and detected the phosphorylations of insulin signaling proteins after acupuncture treatment by using protein chip technology with 720 phosphorylation antibody,and then screened the apoptosis-related proteins which were up-regulated or down-regulated significantly( The increasing of the phosphorylation level ≥ 1. 5 times,decreasing ≤ 0. 67 times). Results( 1) Compared with the sham group,there were 8kinds of apoptosis-related proteins in model group which were increased for ≥1. 5 times,and 4 kinds of apoptosis-related proteins which were decreased for ≤ 0. 67 times;( 2) Compared with the model group,there were 5 kinds of apoptosis-related proteins in the control point group which were increased for ≥1. 5 times,and 8 kinds of apoptosis-related proteins which were decreased for ≤ 0. 67 times; there were 2 kinds of apoptosis-related proteins in acupuncture group which were increased for ≥1. 5 times,and 11 kinds of apoptosis-related proteins which were decreased for ≤ 0. 67 times,there were 3 kinds which were increased in the model group were decreased. Conclusion Brain protection can be realized by inhibiting apoptosis with the regulation of several apoptosis-related proteins after acupuncture treatment on Dazhui,Baihui,Renzhong( acupoints) in CIRI rats.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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