Title: EFFECTS OF STABLY EXPRESSING shRNA TARGETED TO bcl-2 ON MULTIPLICATION OF SGC-7901 CELLS IN GASTRIC CANCER
Abstract: Objective To explore the long-term effect of stable expression shRNA targeted to bcl-2 on SGC-7901 cells in gastric cancer. Methods A shRNA expression vector targeting bcl-2 was created and transfected into SGC-7901 cells,the cells with stable transfection were screened and continuously cultured.The expression of bcl-2 mRNA of SGC-7901 and its effects on cell multiplication and apoptosis were detected by RT-PCR. Results After stable transfection with shRNA,the expression of bcl-2 mRNA in SGC-7901 cells fell dramatically,but the changes of cell reproductive activity and apoptosis were not significant. Conclusion Stable transfection of shRNA has long-term inhibition on expression of bcl-2 mRNA in SGC-7901 cells,which provides an experimental evidence for further study of gene therapy.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot