Title: Conditions of Cellulase Production in the Presence of Microbial Consortia MC1 Capable of Degrading Cellulose with High Efficiency
Abstract: The conditions of cellulase production of microbial consortia MC1 capable of degrading cellulose with high efficiency were investigated with CMC saccharogenic powder and gravimetry. The microbial consortia MC1 could directly degrade carbon source with high native cellulose content (such as filter paper and cotton). It has been found that cellulase activity using peptone and yeast powder as nitrogen source was higher than that using inorganic nitrogen source, such as urea and ammonium nitrate. The optimum fermentation concentrations using filter paper and yeast powder as sole carbon and nitrogen source were found to be 0.5% and 0.125% , respectively, and the highest level of cellulase was discovered at the fifth day after inoculation. The optimum temperature for degrading cellulose was 55 ℃ , but the cellulase activity was inhibited when the temperature was over 60 ℃ .The optimum oxygen concentration of MC1 was 0.01~ 0.04 mg· L- 1 and either higher or lower oxygen concentration was not available to degradation of cellulose. It may be promising and important for the characteristic of microaerobe of MC1 in decreasing the cost of composting engineering.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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Cited By Count: 5
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