Title: Biochemical Characterizations of Acetylcholinesterases of Oxya chinensis in Two Field Populations
Abstract: Acetylcholinesterases (AChE) were characterized and compared in the fifth-instar nymphs of Oxya chinensis from two field populations, collected from Linyi of Shanxi Province and Xuzhou of Jiangsu Province, China. Malathion susceptibility was compared between the two populations by bioassay. Inhibition studies of AChE using three inhibitors, including paraoxon, chlorpyrifos-oxon, demeton-S-methyl, indicated that the Oxya chinensis of Linyi population showed 1.24, 1.13 and 0.82-fold higher susceptibility than that of Xuzhou population respectively, but there were not significant difference between AChE susceptibilities of the two populations. Kinetic studies showed that the Michaelis-Menten constant (Km) and the maximal velocity (Vmax) of AChE from Linyi population were higher than those from Xuzhou population, and the AChE activity in Oxya chinensis of Linyi population were 1.24-fold higher than that of Xuzhou population using acetylthiocholine iodide (ATC) as a substrate, but the difference was not significant. Bioassay results indicated that there existed significant difference in malathion susceptibility between the two populations, and LD50 of the Xuzhou population (13.00 μg·g-1 body weight) was 2.8-fold higher than that of the Linyi population (4.64 μg·g-1 body weight). Therefore, we speculated that AChE might not be correlated to reduced malathion susceptibility of Xuzhou population and there might be some other influence factors.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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Cited By Count: 1
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